Speisky Cosoy Hernán

Profesor Titular
Laboratorio: 
Unidad Académica: 
Teléfono: 
(56) 229781448
Grado Académico: 
PhD. en Farmacología, Universidad de Toronto, Canadá
Químico Farmacéutico, Universidad de Chile
Áreas de Interés: 
Antioxidantes en alimentos
Polifenoles y sus bioactividades
Ingredientes y alimentos funcionales
Reseña: 
El Laboratorio de Antioxidantes (LAOX), constituido y dirigido desde el año 2001 por el Prof. Hernan Speisky, comprende dos espacios altamente equipados que funcionan en forma Independiente. Mientras el primero se centra exclusivamente en la investigación básica y pre-aplicada (realizada por estudiantes de pregrado, magíster, doct- y posdoctorado), el segundo está destinado primariamente a la prestación de servicios de análisis y desarrollo de productos ricos en antioxidantes (ejecutada por profesionales contratados por LAOX). En la actualidad (Oct-2015) el laboratorio dispone de recursos correspondientes a un proyecto Fondecyt, un Innova, un Copec-UC, y un proyecto de posdoctorado. La productividad científica del LAOX supone la publicación de 3-4 trabajos ISI por año. Junto a su labor de investigación, el LAOX realiza una activa labor docente (de pre y postgrado) y de extensión a través de la plataforma digital que LAOX creó y administra www.portalantioxidantes.com
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Publicaciones

2017 Dorta E, Rodríguez E, Fuentes-Lemus E, Speisky H, Lissi E, López-Alarcón C. Oxigen radical absorbance capacity (ORAC) assay to predict the capacity of mango (Mangifera indica L.) by-products to inhibit meat protein oxidation. Food Anal Methods 2017; 10(2):330-338
2017 Suwalsky M, Duguet J, Speisky H. An In Vitro Study of the Antioxidant and Antihemolytic Properties of Buddleja globosa (Matico). J Membr Biol. 2017; 250(3):239-248
2017 Atala E, Fuentes J, Wehrhahn MJ, Speisky H. Quercetin and related flavonoids conserve their antioxidant properties despite undergoing chemical or enzymatic oxidation. Food Chem. 2017; 234:479-485
2016 Aliaga ME, López-Alarcón C, Bridi R, Speisky H. Redox-implications associated with the formation of complexes between copper ions and reduced or oxidized glutathione. J Inorg Biochem 2016; 154:78-88
2016 Sandoval-Acuña C, Fuentes-Retamal S, Guzmán-Rivera D, Peredo-Silva L, Madrid-Rojas M, Rebolledo S, Castro-Castillo V, Pavani M, Catalán M, Maya JD, Jara JA, Parra E, Calaf GM, Speisky H, Ferreira J. Destabilization of mitochondrial functions as a target against breast cancer progression: Role of TPP+-linked-polyhydroxybenzoates. Toxicol Appl Pharmacol. 2016; 309:2-14
2016 Fuentes-Lemus E, Dorta E, Escobar E, Aspee A, Pino E, Abasq ML, Speisky H, Silva E, Lissi E, Davies MJ, López-Alarcón C. Oxidation of free peptide and protein tryptophan residues mediated by AAPH-derived free radicals: role of alkoxyl and peroxyl radicals. RSC Adv. 2016; 6(63):57948-57955
2016 Fuentes J, Montoya P, Vío F, Speisky H. Total phenolics and antioxidant capacity of vegetables grown in the Southwestern Andes Region of South America. J Food Nutr Res 2016; 4(12):760-772
2015 Dorta E, Fuentes E, Aspee A, Atala E, Speisky H, Bridi R, Lissi E, Lopez-Alarcon C. The ORAC (Oxygen Radical Absorbance Capacity) index does not reflect the capacity of antioxidants to trap peroxyl radicals. RSC Adv 2015; 5:39899-39902
2015 Bridi R, Montenegro G, Nuñez-Quijada G, Giordano A, Morán-Romero F, Jara-Pezoa I, Speisky H, Atala E, López-Alarcón C. International regulations of propolis quality: required assays do not necessarily reflect their polyphenolic-related in vitro activities. J Food Sci 2015; 80(6):C1188-C1195
2014 Gotteland M, Cires MJ, Carvallo C, Vega N, Ramirez MA, Morales M, Rivas P, Astudillo F, Navarrete P, Dubos C, Figueroa A, Troncoso M, Ulloa C, Mizgier M l, Carrasco-Pozo C, Speisky H, Brunser O, Figueroa G. Probiotic screening and safety evaluation of Lactobacillus strains from plants, artisanal goat cheese and human stool and milk. J Med Food 2014; 17(4):487-495
2014 Sandoval-Acuña C, Ferreira J, Speisky H. Polyphenols and mitochondria: An update on their increasingly emerging ROS-scavenging independent actions. Arch Biochem Biophys 2014; 559C:75-90
2014 Bridi R, Troncoso MJ, Folch C, Fuentes J, Speisky H, Lopez-Alarcon C A. A polyvinylpolypyrrolidone (PVPP)-assisted Folin-Ciocalteu assay to assess total phenol content of commercial beverages. Food Analytical Methods 2014; 7:2075-2083
2014 Gironés-Vilaplana A, Baenas N, Villaño D, Speisky H, García-Viguera C, Moreno DA. Anti-diabesity potential and antioxidants effects of Latin-American Fruits Rich in Phytochemicals. J Functional Foods 2014; 7: 599-608
2014 Aguilera-Venegas B, Speisky H. Identification of the transition state for fast reactions: The trapping of hydroxyl and methyl radicals by DMPO-A DFT approach. J Mol Graph Model 2014; 52C:57-70
2013 Mura F, Speisky H, Arán V J, Maya J D, Olea-Azara C, Aguilera-Venegas B. Indazoles and quinazolines as potential anti-Trypanosoma cruzi agents: An electrochemical, ESR and biological study. J Spectrosc Dyn 2013; 3:1-8
2013 Aliaga ME, Andrade-Acuña D, López-Alarcón C, Sandoval-Acuña C, Speisky H. Cu(II)-disulfide complexes display simultaneous superoxide dismutase- and catalase-like activities. J Inorg Biochem 2013; 129:119–126
2013 Atala E, Aspée A, Speisky H, Lissi E, López-Alarcón C. Antioxidant capacity of phenolic compounds in acidic medium: a pyrogallol red-based ORAC (Oxygen Radical Absorbance Capacity) assay. J Food Comp Analysis 2013; 32:116-125
2013 Aguilera-Venegas B, Olea-Azar C, Arán V, Speisky H. Indazoles: A new top seed structure in the search of efficient drugs against Trypanosoma cruzi. Future Med Chem 2013; 5(15):1843-1859
2013 Pérez-Cruz F, Cortés C, Atala E, Bohle P, Valenzuela F, Olea-Azar C, Speisky H, Aspée A, Lissi E, López-Alarcón C, Bridi R. Use of pyrogallol red and pyranine as probes to evaluate antioxidant capacities towards hypochlorite. Molecules 2013; 18(2):1638-1652
2013 Atala E, Velázquez G, Vergara C, Mardones CA, Reyes J, Tapia RA, Quina FH, Mendes MA, Speisky H, Lissi E, Ureta-Zañartu MS, Aspee A, López-Alarcón C. Mechanism of pyrogallol red oxidation induced by free radicals and reactive oxidant species. A kinetic and spectroelectrochemistry study. J Phys Chem B 2013; 117(17):4870-4879
2013 Folch-Cano C, Olea-Azar C, Speisky H. Structural and thermodynamic factors on the adsorption process of phenolic compounds onto polyvinylpolypyrrolidone. Colloids and Surfaces A: Physicochem Eng Aspects 2013; 418:105– 111
2013 Folch-Cano C, Guerrero J, Speisky H, Jullian C, Olea-Azar C. NMR and molecular fluorescence spectroscopic study of the structure and thermodynamic parameters of EGCG/β-cyclodextrin inclusion complexes with potential antioxidant activity. J Incl Phenom Macrocycl Chem 2014; 78(1):287-291
2012 Aliaga ME, López-Alarcón C, García-Río L, Martín-Pastor M, Speisky H. Redox-changes associated with the glutathione-dependent ability of the Cu(II)-GSSG complex to generate superoxide. Bioorg Med Chem 2012; 20(9):2869-2876
2012 Speisky H, López-Alarcón C, Gómez M, Fuentes J, Sandoval-Acuña C. First web-based database on total phenolics and oxygen radical absorbance capacity (ORAC) of fruits produced and consumed within the south Andes region of South America. J Agric Food Chem 2012; 80(36):8851-8859
2012 Sandoval-Acuña C, Lopez-Alarcón C, Aliaga ME, Speisky H. Inhibition of mitochondrial complex I by various non-steroidal anti-inflammatory drugs and its protection by quercetin via a coenzyme Q-like action. Chem Biol Interact 2012;199(1):18-28
2012 Ortiz R, Antilén M, Speisky H, Aliaga ME, López-Alarcón C, Baugh S. Application of a microplate-based ORAC-pyrogallol red assay for the estimation ofantioxidant capacity: First Action 2012.03. J AOAC Int 2012; 95(6):1558-1561
2012 Aguilera-Venegas B., Olea-Azar C., Arán V.J., Maya J.D., Kemmerling U., Speisky H., Mendizábal F. Electrochemical, ESR and Theoretical Insights into the Free Radical Generation by 1,1'-Hydrocarbylenebisindazoles and Its Evaluation as Potential Bio-Active Compounds. Int J Electrochem Sci 2012; 7:5837-5863
2012 Carrasco-Pozo C, Mizgier ML, Speisky H, Gotteland M. Differential protective effects of quercetin, resveratrol, rutin and epigallocatechin gallate against mitochondrial dysfunction induced by indomethacin in Caco-2 cells. Chem Biol Interact 2012; 195(3):199-205
2011 Faúndez M, Rojas M, Bohle P, Reyes C, Letelier ME, Aliaga ME, Speisky H, Lissi E, López-Alarcón C. Pyrogallol red oxidation induced by superoxide radicals: Application to evaluate redox cycling of nitro compounds. Anal Biochem 2011; 419(2):284-291
2011 Aliaga ME, Carrasco-Pozo C, López-Alarcón C, Olea-Azar C, Speisky H. Superoxide-dependent reduction of free Fe(3+) and release of Fe(2+) from ferritin by the physiologically-occurring Cu(I)-glutathione complex. Bioorg Med Chem 2011; 19(1):534-541
2011 Speisky H, López-Alarcón C, Olea-Azar C, Sandoval-Acuña C, Aliaga ME. Role of superoxide anions in the redox changes affecting the physiologically occurring cu(i)-glutathione complex. Bioinorg Chem Appl 2011; 2011 article ID 674149 (8 p.)
2011 Ortiz R, Antilén M, Speisky H, Aliaga ME, López-Alarcón C. Analytical parameters of the microplate-based ORAC-pyrogallol red assay. J AOAC Int 2011; 94(5):1562-1566
2011 López-Alarcón C, Gómez M, Lutz M, Speisky H. Time-dependence of ferric reducing antioxidant power (FRAP) index in Chilean apples and berries. Arch Latinoam Nutr 2011; 61(3):323-332
2011 Carrasco-Pozo C, Speisky H, Brunser O, Pastene E, Gotteland M. Apple Peel Polyphenols Protect against Gastrointestinal Mucosa Alterations Induced by Indomethacin in Rats. J Agric Food Chem. 2011; 59: 6459-6466
2011 Carrasco-Pozo C, Gotteland M, Speisky H. Apple peel polyphenol extract protects against indomethacin-induced damage in Caco-2 cells by preventing mitochondrial complex I inhibition. J Agric Food Chem 2011; 59(2):11501-11508
2010 Speisky H, Chiffelle I, Valenzuela T, Araya M, Simpson R, Almonacid S. Development of an Ingredient Containing Apple Peel, as a Source of Polyphenols and Dietary Fibre. J Food Sci 2010; 75(6): H172–H 181
2010 Aliaga ME, López-Alarcón C, Barriga G, Olea-Azar C, Speisky H. Redox-active complexes formed during the interaction between glutathione and mercury and/or copper ions. J Inorg Biochem 2010;104(10):1084-1090
2010 Pastene E, Speisky H, García A, Moreno J, Troncoso M, Figueroa G. In vitro and in vivo effects of apple peel polyphenols against Helicobacter pylori. J Agric Food Chem 2010; 58(12):7172-7179
2010 Aliaga ME, Carrasco-Pozo C, López-Alarcón C, Speisky H. The Cu(I)–glutathione complex: factors affecting its formation and capacity to generate reactive oxygen species. Transition Met Chem 2010; 35(3):321-329
2010 Folch-Cano C, Jullian C, Speisky H, Olea-Azar C. Antioxidant activity of inclusion complexes of tea catechins with β-cyclodextrins by ORAC assays. Food Res Int 2010; 43(8):2039-2044
2010 Henríquez C, Almonacid S, Chiffelle I, Valenzuela T, Araya M, Cabezas L, Simpson R, Speisky H. Determination of antioxidant capacity, total phenolic content and minerals composition of different fruit tissue of five apple cultivars grown in Chile. Chilean J Agricult Res 2010; 70(4):523-536
2010 Carrasco-Pozo C, Gotteland M, Speisky H. Protection by apple peel polyphenols against indomethacin-induced oxidative stress, mitochondrial damage and cytotoxicity in Caco-2 Cells. J Pharm Pharmacol 2010; 62: 943-950
2009 Pastene E, Troncoso M, Figueroa G, Alarcón J, Speisky H. Association between polymerization degree of apple peel polyphenols and inhibition of Helicobacter pylori urease. J Agric Food Chem 2009; 57(2):416-424
2009 Pastene E, Speisky H, Troncoso M, Alarcón J, Figueroa G. In vitro inhibitory effect of apple peel extract on the growth of Helicobacter pylori and respiratory burst induced on human neutrophils. J Agric Food Chem 2009; 57(17):7743-7749
2009 Rodríguez J, Arán VJ, Boiani L, Olea-Azar C, Lavaggi ML, González M, Cerecetto H, Maya JD, Carrasco-Pozo C, Speisky H. New potent 5-nitroindazole derivatives as inhibitors of Trypanosoma cruzi growth: synthesis, biological evaluation, and mechanism of action studies. Bioorg Med Chem 2009; 17(24):8186-8196
2009 Speisky H, Gómez M, Burgos-Bravo F, López-Alarcón C, Jullian C, Olea-Azar C, Aliaga ME. Generation of superoxide radicals by copper-glutathione complexes: Redox-consequences associated with their interaction with reduced glutathione. Bioorg Med Chem 2009; 17(5):1803-1810
2009 Espinoza M, Olea-Azar C, Speisky H, Rodrígues J. Determination of reactions between free radicals and selected Chilean wines and transition metals by ESR and UV-vis technique. Spectrochim Acta A Mol Biomol Spectrosc 2009; 71(5):1638-1643
2009 Atala E, Vásquez L, Speisky H, Lissi E, López-Alarcón C. Ascorbic acid contribution to ORAC values in berry extracts. An evaluation by the ORAC-Pyrogallol red methodology. Food Chem 2009; 113(1): 331-335
2009 Almonacid S, Escobar B, Chiffelle I, Speisky H. Antioxidant content and activity in different structures of five apple cultivars grown in Chile. Acta Horticulturae 2009; 841:275-280
2009 Pastene E, Gomez M, Speisky H. et al. A system for detection of volatile antioxidant commonly emitted from spices and medicinal herbs. Quimica Nova 2009; 32(2): 482-487
2008 Speisky H, Gómez M, Carrasco-Pozo C, Pastene E, Lopez-Alarcón C, Olea-Azar C. Cu(I)-Glutathione complex: a potential source of superoxide radicals generation. Bioorg Med Chem 2008; 16(13):6568-6574
2008 Escobar B, Figuerola F, Chiffelle I, Speisky H, Estévez AM. Characterization of piñon seed (Araucaria araucana (Mol) K. Koch) and the isolated starch from the seed. Food Chem 2008; 107(2):592-601
2008 Speisky H, Peña A, Gómez M, Fredes C, Hurtado M, Gotteland M, Brunser O. Antioxidants in chilean berries. Acta Hort 2008; 777:485-492
2008 Carrasco-Pozo C, Aliaga ME, Olea-Azar C, Speisky H. Double edge redox-implications for the interaction between endogenous thiols and copper ions: In vitro studies. Bioorg Med Chem 2008; 16(22):9795-9803
2008 Carrasco-Pozo C, Gómez M, Brunser O, Speisky H. Slow and fast-reacting antioxidants from berries: Their evaluation through the FRAP (Ferric reducing antioxidant power) assay. Acta Hort 2008; 777:531-536
2008 Gotteland M, Andrews M, Toledo M, Muñoz L, Caceres P, Anziani A, Wittig E, Speisky H, Salazar G. Modulation of Helicobacter pylori colonization with cranberry juice and Lactobacillus johnsonii La1 in children. Nutrition 2008; 24(5):421-426
2007 Muñoz O, Copaja S, Speisky H, Peña R, Montenegro G. Content of flavonoids and phenolic compounds in chilean honeys. Orac index. Quim Nova 2007; 30(4):848-851
2006 Fellenberg MA, Speisky H. Antioxidants: Their effects on broiler oxidative stress and its meat oxidative stability. World’s Poultry Sci J 2006; 62(1):53-70
2006 O'Brien P, Carrasco-Pozo C, Speisky H. Boldine and its antioxidant or health-promoting properties. Chem Biol Interact 2006;159(1):1-17
2006 Speisky H, Rocco C, Carrasco C, Lissi EA, Lopez-Alarcon C. Antioxidant screening of medicinal herbal teas. Phytother Res 2006; 20(6):462-467
2006 Carrasco-Pozo C, Alvarez-Lueje A, Olea-Azar C, López-Alarcón C, Speisky H. In vitro interaction between homocysteine and copper ions: Potential redox implications. Exp Biol Med 2006; 231(9):1569-1575
2005 Lopez-Alarcon C, Rocco C, Lissi E, Carrasco C, Squella JA, Nuñez-Vergara L, Speisky H. Reaction of 5-aminosalicylic Acid with peroxyl radicals: protection and recovery by ascorbic Acid and amino acids. Pharm Res 2005; 22(10):1642-1648
2005 Letelier ME, Pimentel A, Pino P, Lepe AM, Faundez M, Aracena P, Speisky H. Microsomal UDP-glucuronyltransferase in rat liver: oxidative activation. Basic Clin Pharmacol Toxicol 2005; 96(6):480-486
2005 Letelier ME, Lepe AM, Faundez M, Salazar J, Marin R, Aracena P, Speisky H. Possible mechanisms underlying copper-induced damage in biological membranes leading to cellular toxicity. Chem Biol Interact 2005; 151(2):71-82
2004 López-Alarcón C, Speisky H, Squella JA, Olea-Azar C, Camargo C, Núñez-Vergara LJ. Reactivity of 1,4-dihydropyridines toward SIN-1-derived peroxynitrite. Pharm Res 2004; 21(10):1750-1757
2004 Núñez MT, Gallardo V, Muñoz P, Tapia V, Esparza A, Salazar J, Speisky H. Progressive iron accumulation induces a biphasic change in the glutathione content of neuroblastoma cells. Free Radic Biol Med 2004; 37(7):953-960
2004 Santanam N, Penumetcha M, Speisky H, Parthasarathy S. A novel alkaloid antioxidant, Boldine and synthetic antioxidant, reduced form of RU 486, inhibit the oxidation of LDL in vitro and atherosclerosis in vivo in LDLR (-/-) mice. Atherosclerosis 2004; 173(2):203-210
2003 Speisky H, Navarro P, Cherian MG, Jimenez I. Copper-binding proteins in human erythrocytes: searching for potential biomarkers of copper over exposure. Biometals 2003; 16(1):113-123
2003 Araya M, Olivares M, Pizarro F, Gonzalez M, Speisky H, Uauy R. Copper exposure and potential biomarkers of copper metabolism. Biometals 2003; 16(1):199-204
2003 Loghin F, Chagraoui L, Asencio M, Comoy E, Speisky H, Cassels BK, Protais P. Effect of some antioxidative aporphine derivatives on striatal dopaminergic transmission and on MPTP-induced striatal dopamine depletion in B6CBA mice. Eur J Pharm Sci 2003; 18(2):133-140
2003 Araya M, Olivares M, Pizarro F, Gonzalez M, Speisky H, Uauy R. Gastrointestinal symptoms and blood indicators of copper load in apparently healthy adults undergoing controlled copper exposure. Am J Clin Nutr 2003; 77:646-650
2002 Jimenez I, Aracena P, Letelier ME, Navarro P, Speisky H. Chronic exposure of HepG2 cells to excess copper results in depletion of glutathione and induction of metallothionein. Toxicology in Vitro 2002; 16:167-175
2000 Jimenez I, Garrido A, Bannach R, Gotteland M, Speisky H. Protective effect of boldine against free radical-induced erythrocyte lysis. Phytother Res 2000; 14:339-343
2000 Jimenez I, Speisky H. Effects of copper ions on the free radical-scavenging properties of reduced glutathione: implications of a complex formation. Trace Elem Med Biol 2000; 14(3):161-167
2000 Jimenez I, Speisky H. Biological disposition of boldine: in vitro and in vivo studies. Phytother Res 2000; 14(4):254-260
2000 Jimenez I, Lissi E, Speisky H. Free-radical-induced inactivation of lysozyme and carbonyl residue generation in the protein are not necessarily associated. Arch Biochem Biophys 2000; 381(2):247-252
1999 Asencio M, Delaquerriere B, Cassels B, Speisky H, Comoy E, Protais P. Biochemical and behavioral effects of boldine and glaucine on dopamine systems. Pharmacol Biochem Behav 1999; 62:7-13
1998 Olivares M, Pizarro F, Speisky H, Lönnerdal B, Uauy R. Copper in infant nutrition: safety of WHO provisional guideline value for copper content of drinking water. J Pediatr Gastroenterol Nutr 1998; 26:251-257
1998 Hidalgo ME, Gonzalez I, Toro F, Fernandez E, Jimenez I, Speisky H. Boldine as a sunscreen: its photoprotector capacity against UVB radiation. Cosmet Toiletries 1998; 113: 59-66
1998 Mendez E, Sanhueza J, Nieto S, Speisky H, Valenzuela A. Fatty acid composition, extraction, fractionation, and stabilization of bullfrog (rana catesbeiana) oil. JAOCS 1998; 75:67-71
1998 Ganga A, Nieto S, Sanhueza J, Romo C, Speisky H, Valenzuela A. Concentration and stabilization of n-3 polyunsaturated fatty acids from sardine oil. JAOCS 1998; 75:733-736
1997 Jimenez I, Gotteland M, Zarzuelo A, Uauy R, Speisky H. Loss of the metal binding properties of metallothionein induced by hydrogen peroxide and free radicals. Toxicology 1997; 120:37-46
1997 Gotteland M, Jimenez I, Brunser O, Guzman L, Romero S, Cassels B, Speisky H. Protective effects of boldine in experimental colitis. Planta Med 1997; 63:311-315
1997 Aposhian V, Arroyo A, Cebrian M, Del Razo LM, Hurlbult KH, Dart RC, Gonzalez-Ramirez D, Kreppel H, Speisky H, Smith A, Gonsebatt ME, Ostrovsky-Wegman P, Aposhian MM. DMPS-arsenic challenge test: l-increased urinary excretion of monomethylarsonic acid in humans given dimercaptopropane sulphonate. J Pharmacol Exp Ther 1997; 282(1):192-200
1997 Mendez E, Sanhueza J, Speisky H, Valenzuela A. Comparison of two Rancimat evaluation modes in the assessment of the relative stability of fish oil. J Am Oil Chem Soc 1997; 74:331-332
1996 Luza S, Speisky H. Liver copper storage and transport during development: implications for citotoxicity. Am J Clin Nutr 1996; 63:812S-820S
1996 Mendez E, Sanhueza J, Speisky H, Valenzuela A. Validation of the Rancimat test for the assessment of the relative stability of fish oils. J Am Oil Chem Soc 1996; 73:1033-1037
1996 Bannach R, Valenzuela A, Cassels BK, Nuñez-Vergara L, Speisky H. Cytoprotective and antioxidant effects of boldine on tert-butyl hydroperoxide-induced damage to isolated hepatocytes. Cell Biol Toxicol 1996; 12:89-100
1995 Cassels B, Asencio M, Conget P, Speisky H, Videla L, Lissi E. Structure-antioxidative activity relationships in benzylisoquinoline alkaloids. Pharmacol Res 1995; 31:103-107
1995 Hu J, Speisky H, Cotgreave IA. The inhibitory effects of boldine, glaucine and probucol on TPA-induced down regulation of gap junction function: Relationships to intracellular peroxides, protein kinase C, translocation and connexin 43 phospholiration. Biochem Pharmacol 1995; 50(10):1635-1643
1994 Speisky H, Cassels B. Boldo and boldine: An emerging case of natural drug development. Pharmacol Res 1994; 29(1):1-12
1994 Morello A, Lipchenca J, Cassels B, Speisky H, Aldunate J, Repetto Y. Trypanocidal effect of boldine and related alkalids upon several strains of trypanosoma Cruzi. Comp Biochem Physiol 1994; 107C(3):367-371
1994 Gonzalez-Cabello R, Speisky H, Bannach R, Valenzuela A, Feher J, Gergely P. Effects of boldine on cellular immune functions in vitro. J Invest Allergol Clin Immunol 1994; 4(3):139-145
1994 Hirai M, Iwamura S, Varghese G, Speisky H, Israel Y. Reciprocal gamma-glutamyl transferase and cystathionase activity in guinea pig, rat and human liver. J Hepatol 1994; 21:683-688
1994 Backhouse N, Del Porte C, Givemau M, Cassels BK, Valenzuela A, Speisky H. Anti-inflammatory and anti-pyretic effects of boldine. Agents Actions 1994; 42:114-117
1993 Speisky H, Valenzuela A, Nieto S, Cassels B, Nuñez-Vergara L. Determination of boldine in plasma by high performance liquid chromatography. J Chromatogr 1993; 612(2):315-319
1993 Asencio M, Cassels B, Speisky H, Valenzuela A. (R)-and (S)-Coclaurine from the bark of Peumos boldus. Fitoterapia 1993; 64(5):455-458
1992 Speisky H. Age-dependent removal of circulating glutathione by the perfused rat liver: Role of gamma-glutamyl transferase. Age 1992; 15:104-107
1992 Shackel NA, Speisky H, Wade DN. Hepatic glutathione degradation within the sinusoids of guinea pig livers shows a periportal localization of gamma glutamyl-transferase activity. Agents Actions 1992; 36(3-4):289-294
1992 Cederbaum AI, Kukielka E, Speisky H. Inhibition of rat liver microsomal lipid peroxidation by boldine. Biochem Pharmacol 1992; 44(9):1765-1772
1991 Nuñez-Vergara L, Yudelevich J, Squella A, Speisky H. Drug-acetaldehyde interactions during ethanol metabolism in vitro. Alcohol Alcohol 1991; 26:139-144
1991 Speisky H, Cassels B, Lissi E, Videla L. Antioxidant properties of the alkaloid boldine in systems undergoing lipid peroxidation and enzyme inactivation. Biochem Pharmacol 1991; 41:1575-1581
1991 Cameron R, Armstrong D, Gunasekara A, Varghese G, Speisky H. Utilization of circulating GSH by nodular and cancerous intact rat liver. Carcinogenesis 1991; 12:2369-2372
1991 Speisky H, Squella A, Nuñez-Vergara L. Activity of boldine on rat ileum. Planta Medica 1991; 57(6):519-522
1991 Valenzuela A, Nieto S, Cassels B, Speisky H. Inhibitory effect of boldine on fish oil oxidation. J Am Oil Chem Soc 1991; 68(12):935-937
1990 Turner CJ, Bhatnagar MK, Speisky H. Effect of subchronic administration of ethanol and methylmercury in combination on the tissue distribution of mercury in rats. Can J Physiol Pharmacol 1990; 68(12):1558-1562.
1990 Speisky H, Israel Y. Gamma-glutamyl transferase ectoactivity in the intact rat liver: effect of chronic alcohol consumption. Alcohol 1990; 7(4):339-347
1990 Speisky H, Shackel N, Varghese G, Wade D, Israel Y. Role of hepatic gamma-glutamyltransferase in the degradation of circulating glutathione: studies in the intact guinea pig perfused liver. Hepatology 1990; 11(5):843-849
1989 Lindros KO, Penttilä KE, Gaasbeek Janzen JW, Moorman AF, Speisky H, Israel Y. The gamma-glutamyltransferase/glutamine synthetase activity ratio. A powerful marker for the acinar origin of hepatocytes. J Hepatol 1989; 8(3):338-343
1988 Speisky H, Kera Y, Penttilä KE, Israel Y, Lindros KO. Depletion of hepatic glutathione by ethanol occurs independently of ethanol metabolism. Alcohol Clin Exp Res 1988; 12(2):224-228
1987 Kera Y, Speisky H, Penttilä KE, Sippel H, Lindros KO. Glutathione metabolism in isolated rat hepatocytes: acinar heterogeneity of detoxifying enzymes and effects of ethanol. Alcohol Alcohol 1987; 1 Suppl:235-239
1987 Speisky H, Gunasekara A, Varghese G, Israel Y. Basolateral gamma-glutamyl transferase ectoactivity in rat liver: effects of chronic alcohol consumption. Alcohol Alcohol 1987; 1 Suppl:245-249
1985 Speisky H, MacDonald A, Giles G, Orrego H, Israel Y. Increased loss and decreased synthesis of hepatic glutathione after acute ethanol administration. Turnover studies. Biochem J 1985; 225(3):565-572
1985 Speisky H, Bunout D, Orrego H, Giles HG, Gunasekara A, Israel Y. Lack of changes in diene conjugate levels following ethanol induced glutathione depletion or hepatic necrosis. Res Comm Chem Pathol Pharmacol 1985; 48(1):77-90
2007 López-Alarcón C, Speisky H, Lissi E. Antioxidant effect of 5-amino salicylic acid on copper-mediated LDL oxidation. Biol Res 2007; 40(2):155-162
2000 Speisky H, Jimenez I. Radicales libres y antioxidantes en la prevención de enfermedades(I): Mecanismos de generación de radicales libres (Free radicals and antioxidants in disease prevention: (I) Free radical-generating mechanisms). Rev Chil Nutr 2000; 27(1):48-55
2000 Jimenez I, Speisky H. Radicales libres y antioxidantes en la prevención de enfermedades (II): Mecanismos de defensa antioxidante (Free radicals and antioxidants in disease prevention: (II) antioxidant defense mechanisms). Rev Chil Nutr 2000; 27(2):210-219
2000 Speisky H, Jimenez I. Radicales libres y antioxidantes en la prevención de enfermedades (III): Evidencias clínico-epidemiológicas de los riesgos y beneficios asociados al consumo de antioxidantes en la prevención de enfermedades cardiovasculares (Free radicals and antioxidants in disease prevention (III): clinical and epidemiological evidences of the risks and benefits associated with antioxidant consumption in cardiovascular diseases prevention). Rev Chil Nutr 2000; 27(3):314-325
1995 Gotteland M, Espinoza J, Cassels B, Speisky H. Efecto de un extracto seco de boldo sobre el tránsito intestinal en voluntarios sanos (Effect of a dry boldo extract on oro cecal intestinal transit time in healthy volunteers). Rev Med Chil 1995; 123:955-960
1995 Speisky H, Squella JA, Nuñez-Vergara LJ. Implicancias farmacocinéticas asociadas al uso de fármacos como racematos o enantiómeros puros (Pharmacokinetic implications associated to the use of drugs as racemates or pure). Rev Med Chil 1995; 123:884-891
1993 Asencio M, Cassels B, Speisky H. The methylation of boldine with diazomethane. Bol Soc Chil Quim 1993; 38:331-334
2016 Zacarías I, Speisky H, Fuentes J, González CG, Domper A, Fonseca L, Olivares S. Los colores de la salud. 3 Verduras y 2 Frutas al día (The colors of health. 3 Vegetables and 2 fruits a day). Santiago: Ministerio de Agricultura; Corporación 5 al día; Universidad de Chile, INTA; 2016
2016 Speisky H, Fuentes J. Antioxidantes en verduras y frutas (Antioxidants in vegetables and fruits). En: Zacarías I, Speisky H, Fuentes J, González CG, Domper A, Fonseca L, Olivares S. Los colores de la salud. 3 Verduras y 2 Frutas al día. Santiago: Ministerio de Agricultura; Corporación 5 al día; Universidad de Chile, INTA; 2016, p.27-35
2016 Speisky H, Fuentes J, Dorta E, López-Alarcón C. Polyphenols: Sources and main characteristics. In: Cuevas-Valenzuela J, Vergara-Salinas JR, Pérez-Correa JR., editors. Advances in technologies for producing food-relevant polyphenols. Boca Raton, FL: CRC Press; 2016, p. 1-32
2008 Speisky H. Frutas y verduras como fuentes de antioxidantes naturales: Su importancia para la salud humana (Fruits and vegetables as sources of natural antioxidants: Its role for human health). En:Chile. Ministerio de Agricultura; Universidad de Chile, INTA; Corporación 5 al Día. Contribución de la política agraria al consumo de frutas y verduras en Chile: Un compromiso con la nutrición y la salud de la población. Santiago: Maval Ltda., 2008. p.45-60
1992 Israel Y, Speisky H, Lanca AJ, Iwamura S, Hirai M, Varghese G. Metabolism of hepatic glutathione and its relevance in alcohol induced liver damage. In: Clement B, Guillouzo A., editors. Cellular and molecular aspects of cirrhosis. Paris: INSERM; 1992. p.25-37 (Colloque INSERM; 216)
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