Cargo: Profesor titular
Ver perfil Uchile

His present interest is Genomic Evolution of Vibrio parahaemolyticus in the Ocean. Internationally known for his contributions in Microbiology, Biotechnology, and food associated infectious diseases.  He has more than 100 ISI publications with more than 3,800 citations (  Professor Espejo is member of the American Academy of Microbiology, the Chilean Academy of Sciences and  has participated in several international committees related to food and safety; Steering Committee, “Scientific working group on viral diarrheas: Microbiology, epidemiology, immunology and vaccine development”, The Diarrhoeal Disease Control Programme, WHO, Permanent Committee for Laboratory Aspects of Fight against Diarrhoeal Disease, Panamerican Health Association. Joint FAO/WHO,  Expert Meetings on Microbiological Risk Assessment (JEMRA)”

Grado Académico
  • Bioquímico
Unidad Académica


Áreas de interés
  • Microbiología genómica
2010 González-Escalona N, Moreno CX, Romero J, Espejo RT. Vibrio parahaemolyticus strain ATCC 17802 contains two transcriptionally active 16S rRNA genes and neither degrades preferentially in starvation. In: Méndez-Vilas A., editor. Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology. Badajoz, España:Formatex Research Center; 2010 (Microbiology Series;2, Vol. 1:754-757
1995 Vasquez M, Espejo RT. Selection of bacteria originally present in bioleaching system upon laboratory culturing in different media. In: Vargas T, Jerez C, Wiertz J, Toledo H., editors. Biohydrometallurgical processing. Santiago: University of Chile; 1995. p. 135-142
1995 Espejo RT, Pizarro J, Jedliki E, Orellana O, Romero J. Bacterial populations in the bioleaching of copper as revealed by analysis of DNA obtained from leached ores and leaching solutions. In: Jerez CA, Vargas T, Toledo H, Wiertz JV., editors. Biohyhrometallurgical Processing. Santiago:University of Chile Press; 1995. v.II, p.1-8
2017 Timmis K, Jebok F, Espejo RT. Microbiome yarns: microbial forensics for auditing provenance in global food chains. Microb Biotechnol 2017; 10(4):678-682
2017 García K, Yáñez C, Plaza N, Peña F, Sepúlveda P, Pérez-Reytor D, Espejo RT. Gene expression of Vibrio parahaemolyticus growing in laboratory isolation conditions compared to those common in its natural ocean environment. BMC Microbiol. 2017 May 19;17(1):118
2017 Espejo RT, García K, Plaza N. Insight into the origin and evolution of the Vibrio parahaemolyticus pandemic strain. Front Microbiol. 2017 Jul 24;8:1397
2017 Pérez-Reytor D, Plaza N, Espejo RT, Navarrete P, Bastías R, Garcia K. Role of non-coding regulatory RNA in the virulence of human pathogenic Vibrios. Front Microbiol. 2017 Jan 11; 7:2160
2016 Castillo D, Christiansen RH, Dalsgaard I, Madsen L, Espejo R, Middelboe M. Comparative genome analysis provides insights into the pathogenicity of Flavobacterium psychrophilum. PLoS One 2016 Apr 12; 11(4):e0152515
2015 Loyola DE, Navarro C, Uribe P, García K, Mella C, Díaz D, Valdes N, Martínez-Urtaza J, Espejo RT. Genome diversification within a clonal population of pandemic Vibrio parahaemolyticus seems to depend on the life circumstances of each individual bacteria. BMC Genomics 2015;16:176
2015 Yáñez R, Bastías R, Higuera G, Salgado O, Katharios P, Romero J, Espejo R, García K. Amplification of tlh gene in other Vibrionaceae specie by specie-specific multiplex PCR of Vibrio parahaemolyticus. Electron J Biotechnol 2015; 18:459-463
2015 García K, Ramírez-Araya S, Díaz A, Reyes-Cerpa S, Espejo RT, Higuera G, Romero J. Inactivated E. coli transformed with plasmids that produce dsRNA against infectious salmon anemia virus hemagglutinin show antiviral activity when added to infected ASK cells. Front Microbiol 2015; April 16; 6:300
2014 Castillo D, Christiansen RH, Espejo R, Middelboe M. Diversity and geographical distribution of flavobacterium psychrophilum isolates and their phages: patterns of susceptibility to phage infection and phage host range. Microb Ecol 2014; 67(4):748-757
2014 Romero J, Higuera G, Gajardo F, Castillo D, Middleboe M, García K, Ramírez C, Espejo RT. Complete genome sequence of Vibrio anguillarumphage CHOED successfully used for phage therapy in aquaculture. Genome Announc 2014; 2(4):e00091-14
2014 Castillo D, Espejo R, Middelboe M. Genomic structure of bacteriophage 6H and its distribution as prophage in Flavobacterium psychrophilum strains. FEMS Microbiol Lett 2014;351(1):51-58
2013 García K, Bastías R, Higuera G, Torres R, Mellado A, Uribe P, Espejo RT. Rise and fall of pandemic Vibrio parahaemolyticus serotype O3:K6 in southern Chile. Environ Microbiol 2013; 15(2):527-534
2013 Higuera G, Bastías R, Tsertsvadzeb G, Romero J, Espejo R. Recently discovered Vibrio anguillarum phages can protect against experimentally induced vibriosis in Atlantic salmon, Salmo salar. Aquaculture 2013; 392-395:128-133
2013 Navarrete P, Fuentes P, de la Fuente L, Barros L, Magne F, Opazo R, Ibacache C, Espejo RT, Romero J. Short-term effects of dietary soybean meal and lactic acid bacteria on the intestinal morphology and microbiota of Atlantic salmon (Salmo salar). Aquacult Nutr 2013; 19:827-836
2012 Castillo D, Higuera G, Villa M, Middelboe M, Dalsgaard I, Madsen L, Espejo RT. Diversity of Flavobacterium psychrophilum and the potential use of its phages for protection against bacterial cold water disease in salmonids. J Fish Dis 2012;35(3):193-201
2012 García K, Gavilán RG, Höfle MG, Martínez-Urtaza J, Espejo RT. Microevolution of Pandemic Vibrio parahaemolyticus assessed by the number of repeat units in short sequence Tandem repeat regions. PLoS One 2012;7(1):e30823
2012 Navarrete P, Magne F, Araneda C, Fuentes P, Barros L, Opazo R, Espejo R, Romero J. PCR-TTGE analysis of 16S rRNA from rainbow trout (Oncorhynchus mykiss) gut microbiota reveals host-specific communities of active bacteria. PLoS One 2012;7(2):e31335
2012 Opazo R, Ortúzar F,Navarrete P, Espejo R, Romero J. Reduction of oybean meal non-starch polysaccharides and α-galactosides by solid-state fermentation using cellulolytic bacteria obtained from different environments. Plos One 2012; 7(9):e44783
2010 Bastías R, Higuera G, Sierralta W, Espejo RT. A new group of cosmopolitan bacteriophages induce a carrier state in the pandemic strain of Vibrio parahaemolyticus. Environ Microbiol. 2010 Apr;12(4):990-1000
2010 Ansede-Bermejo J, Gavilan RG, Triñanes J, Espejo RT, Martinez-Urtaza J. Origins and colonization history of pandemic Vibrio parahaemolyticus in South America. Mol Ecol 2010; 19(18):3924-3937
2010 Navarrete P, Toledo I, Mardones P, Opazo R, Espejo RT, Romero J. Effect of Thymus vulgaris essential oil on intestinal bacterial microbiota of rainbow trout, Oncorhynchus mykiss (Walbaum) and bacterial isolates. Aquacult Res 2010; 41:e667-e678
2009 Harth-Chu E, Espejo RT, Christen R, Guzmán CA, Höfle MG. Multiple-locus variable-number tandem-repeat analysis for clonal identification of Vibrio parahaemolyticus isolates by using capillary electrophoresis. Appl Environ Microbiol 2009; 75(12):4079-4088
2009 Zabala B, Hammerl JA, Espejo RT, Hertwig S. The linear plasmid prophage Vp58.5 of Vibrio parahaemolyticus is closely related to the integrating phage VHML and constitutes a new incompatibility group of telomere phages. J Virol 2009; 83(18):9313-9320
2009 Zabala B, García K, Espejo RT. Enhancement of Vibrio parahaemolyticus O3:K6 pandemic. Appl Environ Microbiol 2009; 75(6):1697-1702
2009 Navarrete P, Espejo RT, Romero J. Molecular analysis of microbiota along the digestive tract of juvenile Atlantic Salmon (Salmo salar L.). Microb Ecol 2009; 57(3):550-561
2009 Harth E, Matsuda L, Hernández C, Rioseco ML, Romero J, González-Escalona N, Martínez-Urtaza J, Espejo RT. Epidemiology of Vibrio parahaemolyticus outbreaks, southern Chile. Emerg Infect Dis 2009; 15(2):163-168
2009 García K, Torres R, Uribe P, Hernández C, Rioseco ML, Romero J, Espejo RT. Dynamic of clinical and environmental Vibrio parahaemolyticus during seafood-related summer diarrhea outbreaks in southern Chile. Appl Environ Microbiol 2009; 75(23):7482-7487
2008 Navarrete P, Mardones P, Opazo R, Espejo R, Romero J. Oxytetracycline treatment reduces bacterial diversity of intestinal microbiota of Atlantic salmon. J Aquat Anim Health 2008; 20(3):177-183
2008 González-Escalona N, Martínez-Urtaza J, Romero J, Espejo RT, Jaykus LA, DePaola A. Determination of molecular phylogenetics of Vibrio parahaemolyticus strains by multilocus sequence typing. J Bacteriol 2008; 190(8):2831-2840
2007 Fuenzalida L, Armijo L, Zabala B, Hernández C, Rioseco ML, Riquelme C, Espejo RT. Vibrio parahaemolyticus strains isolated during investigation of the summer 2006 seafood related diarrhea outbreaks in two regions of Chile. Int J Food Microbiol 2007; 117(3):270-275
2007 Cabello FC, Espejo RT, Hernandez MC, Rioseco ML, Ulloa J, Vergara JA. Vibrio parahaemolyticus O3:K6 epidemic diarrhea, Chile, 2005. Emerg Infect Dis 2007; 13(4):655-656
2007 Harth E, Romero J, Torres R, Espejo RT. Intragenomic heterogeneity and intergenomic recombination among Vibrio parahaemolyticus 16S rRNA genes. Microbiology 2007; 153(Pt 8):2640-2647
2006 González-Escalaona N, Romero J, Guzmán CA, Espejo RT. Variation in the 16S-23S rRNA intergenic spacer regions in Vibrio parahaemolyticus strains are due to indels nearby their tRNAGlu. FEMS Microbiol Lett 2006; 256:38-43
2006 Fuenzalida L, Hernández C, Toro J, Romero J, Espejo RT. Vibrio parahaemolyticus in shellfish and clinical samples during two large epidemics of diarrhea in Southern Chile. Environ Microbiol 2006; 8:675-683
2005 Cabello A, Espejo RT, Romero J. Tracing Vibrio parahaemolyticus in oysters (Tiostrea chilensis) using a Green Fluorescent Protein tag. J Exp Marine Biol Ecol 2005; 327:157-166
2005 Gonzalez-Escalona N, Romero J, Espejo RT. Polymorphism and gene conversion of the 16S rRNA genes in the multiple rRNA operons of Vibrio parahaemolyticus. FEMS Microbiol Lett 2005; 246(2):213-219
2005 Gonzalez-Escalona N, Cachicas V, Acevedo C, Rioseco ML, Vergara JA, Cabello F, Romero J, Espejo RT. Vibrio parahaemolyticus diarrhea, Chile, 1998 and 2004. Emerg Infect Dis 2005; 11(1):129-131
2003 Romero J, Yanez C, Vasquez M, Moore RB, Espejo R. Characterization and identification of an iron-oxidizing Leptospirullum-like bacterium, present in the high sulfate leaching solution of a commercial bioleaching plant. Res Microbiol 2003; 143:353-359
2003 Gonzalez N, Romero J, Espejo RT. Comprehensive detection of bacterial populations by PCR amplification of the 16S-23S rRNA spacer region. J Microbiol Methods 2003; 55(1):91-97
2002 Romero J, Gonzalez N, Espejo RT. A marine pseudoalteromonas sp. composes most of the bacterial population developed in oysters (Tiostrea chilensis) spoiled during storage. J Food Sci 2002; 67(6):2300-2303
2002 Moreno C, Romero J, Espejo RT. Polymorphism in repeated 16S rRNA genes is a common property of type strains and environmental isolates of the genus Vibrio. Microbiology 2002; 148:1233-1239
2002 Romero J, Garcia-Varela M, Laclette JP, Espejo RT. Bacterial 16S rRNA gene analysis revealed that bacterial related to Arcobacter spp. constitute an abundant and common component of the oyster microflora (Tiostrea chilensis). Microb Ecol 2002; 44:365-371
2001 Romero J, Espejo RT. The prevalence of non- cultivable bacteria in oysters (Tiostrea chilensis, philippi 1845). J Shellfish Res 2001; 20:1235-1240
1999 Vasquez M, Moore ERB, Espejo RT. Detection by polymerase chain reaction-amplification and sequencing of an archaeon in a commercial-scale copper bioleaching plant. FEMS Microbiol Lett 1999; 173:183-187
1999 Uribe P, Suarez Isla BA, Espejo RT. Ribosomal RNA heterogeneity and identification of toxic dinoflagellates cultures by heteroduplex mobility assay. J Phycol 1999; 35:884-888
1998 Espejo RT, Feijoo CG, Romero J, Vasquez M. PAGE analysis of the heteroduplexes formed between PCR amplified 16S Ribosomal RNA genes: estimation of sequence similarity and rDNA complexity. Microbiology 1998; 144:1611-1617
1997 Espejo RT, Romero J. Bacterial community in copper sulfide ores inoculated and leached with solution from a commercial-scale copper leaching plant. Appl Environ Microbiol 1997; 63:1344-1348
1997 Vasquez M, Espejo RT. Chemolithotrophic bacteria in copper ores leached at high sulfuric acid concentrations. Appl Environ Microbiol 1997; 63:332-334
1996 Pizarro J, Jedlicki E, Orellana O, Romero J, Espejo RT. Bacterial population in samples of bioleached copper ore as revealed by analysis of DNA obtained before and after cultivation. Appl Environ Microbiol 1996; 62:1323-1328
1993 Espejo RT, Escanilla D. Detection of HIV-1 DNA by a simple procedure of polymerase chain reaction, using primer-dimer” formation as an internal control of amplification. Res Virol 1993; 144:243-246
1993 Ramirez E, Uribe P, Escanilla D, Sanchez G, Espejo RT. Reactivity patterns and infection status of serum samples with indeterminate Western immunoblot tests for antibody to hunan immunodeficiency virus type 1. J Clin Microbiol 1993; 30:801-805
1991 Huilan S, Zhen LG, Mathan MM, Mathew MM, Olarte J, Espejo R, Khin Maung U, Ghafoor MA, Khan MA, Sami Z, et al. Etiology of acute diarrhoea among children in developing countries: a multicentre study in five countries. Bull World Health Organ 1991; 69(5):549-555
1990 Espejo RT, Uribe P. Immunoprecipitation of Human Immunodeficiency virus type 2 glycoproteins by sera positive for human immunodeficiency virus type 1. J Clin Microbiol 1990; 28:2107-2111
2004 Acevedo C, Romero J, Espejo R. Actividad de distintas presentaciones comerciales de Saccharomyces boulardii (Camparative activity of different commercial products containing Saccharomyces boulardii). Rev Chil Nutr 2004; 31(1):33-38
1995 Gaggero A, Escanilla D, Larrañaga C, Uribe P, Espejo RT. Progreso de la infección viral en mellizos hijos de madre infectada por el virus de la inmunodeficiencia humana (Progression of viral infection in two twins born from a mother infected with the human immunodeficiency virus). Rev Méd Chil 1995; 123:1189-1198

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